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Embryonic brain samples were fixed overnight in 4% paraformaldehyde (PFA).
Brain samples were fixed in 10% buffered formalin, dehydrated, and embedded in paraffin wax for examination for pathology.
For histopathology, the brain samples were fixed in 10% neutral buffered formalin, embedded in paraffin and 6-µm vertical sections were stained with hematoxylin and eosin.
Following cardiac perfusion with 4% PFA, brain samples were fixed with 2.0% PFA, 2.5% glutaraldehyde and 0.1 M cacodylate buffer (pH 7.4), and postfixed with 1% osmium tetroxide followed by 1% uranyl acetate, dehydrated through a graded series of ethanol and embedded in LX112 resin (LADD Research Industries, Burlington VT).
Half brain samples were fixed in formaldehyde before analysis.
Ex vivo whole mouse brain samples were fixed in formaldehyde and placed in fomblin (Sigma) immediately prior to imaging.
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Brain tumour samples were fixed in 10% neutral-buffered formalin (Sigma) and embedded in paraffin.
Brain tissue samples were fixed in 2.5% glutaraldehyde/2% paraformaldehyde in cacodylate buffer, rinsed in buffer and post fixed in 2% osmium tetroxide for 1 h.
In addition, liver, kidney, brain, lung, spleen, and heart tissue samples were fixed and preserved in 4% buffered formaldehyde for at least 24 h.
For the measurement of brain volume, adult heads were collected, the probosces were removed, and the samples were fixed in 4% paraformaldehyde in PBS overnight.
Samples were fixed in 4% paraformaldehyde overnight.
More suggestions(16)
brain explants were fixed
brain biopsies were fixed
brain samples were isolated
brain samples were deproteinized
brain samples were quantified
brain samples were measured
brain samples were included
brain samples were weighed
brain samples were used
brain samples were shipped
brain samples were performed
brain slides were fixed
brain samples were examined
brain samples were postfixed
brain samples were analyzed
brain samples were homogenized
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