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After perfusion, brain samples were dissected and split into two parts by coronally cutting into 2 mm pieces; one part was processed for tissue clearing method as described below, the other part was incubated in 20% sucrose-PBS until samples sank at the bottom of the tube, embedded in OCT compound (Tissue-Tek®) and stored at −80 °C until sectioning.
Brain samples were dissected in phosphate-buffered saline (PBS) and fixed in 4% paraformaldehyde for 30 min at room temperature.
Brain samples were dissected from mice, flash frozen in liquid nitrogen, and stored at −80°C until processed.
Brain samples were dissected by an experienced neuropathologist (median: 20.5 hours (min.7 hours – max.37 hours) after death) and deep-frozen immediately after collection.
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Forebrains were cut into 140 μm thick coronal sections with a Vibroslicer (Leica Microsystems VT1000S) from where samples were dissected of respective brain areas and cryoprotected with 30%% glycerol in 0.1 M PB overnight at 6 °C.
Brains were rapidly removed, and striatum and forebrain samples were dissected and placed in ice-cold 0.2 N perchloric acid and frozen at −80°C.
DFC tissue samples were dissected from postmortem adult chimpanzee and macaque brains using the criteria previously described [ 23, 24].
Tissue samples were dissected from the prefrontal cortex (Brodmann area 9) of two healthy control human brain samples in order to provide sufficient confidence in protein identifications.
Samples were dissected as described above for the BrdU assay.
Samples were dissected the same day as the radiological scans.
Tissue samples were dissected and flash-frozen in liquid nitrogen.
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