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Brain samples were cleared in FocusClear™ (CelExplorer, Taiwan) for 5 min, mounted in a drop of MountClear™ (CelExplorer, Taiwan) and then imaged under a Zeiss LSM 510 confocal microscope [86].
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Samples were cleared in 70% ethanol and photographed.
After wiping with Product T, samples were cleared of contamination.
Finally, after extensive washing, the immunolabeled brain samples were directly cleared in FocusClear (CelExplorer, Taiwan) for 5 min and then mounted in a drop of MountClear (CelExplorer) and then imaged under a Zeiss LSM 510 confocal microscope.
Visually adhesive samples were clear and colourless.
The brain samples were deproteinized by acetonitrile with IS.
Embryonic brain samples were fixed overnight in 4% paraformaldehyde (PFA).
Fetal and adult brain samples were lyzed using RIPA buffer.
Blood and brain samples were never taken for research.
Brain samples were homogenized using a Virsonic 100 ultrasonic homogenizer.
Brain samples were collected at the end of the experiment.
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