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The above data highlighted the expression of HERV-W env in adult human brain in the context of disease, but to gain more insight into its expression, three fetal (F1-3) and two adult (A1-2) brain samples were assessed by deep sequencing, which yielded multiple HERV-W RNA-derived sequence tags.
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Serial sections from the paraffin-embedded brain tissue samples were assessed for neuropathology blind to clinical status according to the CERAD protocol [ 2, 3] and Braak stage [ 4, 5].
Samples were assessed in duplicate.
Spleen samples were assessed by immunohistochemical staining.
Samples were assessed for viable tumour.
Samples were assessed with no prior purification.
Samples were assessed microbiologically and cytologically.
All samples were assessed in duplicates.
Samples were assessed as described previously.
At the end of experiment, blood, liver and brain samples were collected to assess biochemical and biophysical markers as well histopathological investigations.
The quality of each of these brain samples was previously assessed by determining the RNA Integrity Number RINN) using an Agilent 2100 Bioanalyzer, and only samples with RIN values >6 were included in this study.
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