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Three independent samples, each from two pooled adult brain samples were analyzed for each genotype.
Both plasma and brain samples were analyzed using LC/MS/MS determination, according to ADME-Bioanalysis (Vergeze, France) procedures.
In a second step, mRNA expression from controls and ARTE10 mouse brain samples were analyzed in parallel.
The sequence tags derived from deep sequencing of fetal and adult (healthy) brain samples were analyzed for the abundance of different host immune genes.
To determine if the FE-Pro complexes were well-retained and encapsulated within NSCs after implantation into the mouse brain and subsequent tumor-tropic migration, the brain samples were analyzed with TEM and immunohistochemistry.
The residual lipids extracted from the paraffin embedded brain samples were analyzed using a procedure recently described [ 13].
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Brain tissue samples were analyzed by ELISA for SHH.
Quantitative data are expressed as the mean ± 1 S.E.M. Mechanically dissociated and fixed adult rat brain tissue samples were analyzed by FCM.
The RNF138 mRNA expression levels of 35 pathological confirmed glioma samples (Grade I – 4 cases, Grade II – 13 cases, Grade III – 11 cases, and Grade IV – 7 cases) and five noncancerous brain tissue samples were analyzed by real-time quantitative PCR.
The brains from the positive samples were analyzed by real-time PCR using primers BoHV F (TAC GGA CTG CCG GAT TAA CA) and BoHV R (GTC ACC CTA CCA CCG CCG CCA ACA T), according to the protocol of Pedraza-Ordoñez et al. [ 19].
For allelic-imbalance (AI) analysis, 77 primary breast tumor (55 fresh-frozen and 22 paraffin-embedded samples) and 21 brain metastases (all fresh-frozen) samples were analyzed.
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