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The homogenized brain samples were imaged under 60x magnification.
Brain samples were processed as described in detail before51.
Brain samples were serially sectioned at the striatal region.
The brain samples were deproteinized by acetonitrile with IS.
Western blot analyses of brain samples were performed as described in33.
Rat brain samples were homogenized in the same buffer used for the human brain samples.
A total of 52 brain samples were included in the present study.
All the mice were sacrificed on day 14 and brain samples were processed for neurochemical, biochemical and immunohistochemistry studies.
The brain samples were obtained from The Netherlands Brain Bank, Netherlands Institute for Neuroscience, Amsterdam (open access: www.brainbank.nl).nl
Brain samples were postfixed with 4% PFA overnight and equilibrated in 15% and 30% sucrose.
Brain samples were first evaluated by standard hematoxylin and eosin (H&E) staining.
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