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Accumulation of amyloid-β1-42 rands and τ filaments in necropsied brain samples was verified with the help of ELISA.
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Sex- and age-matching of both sub samples was verified.
DNA samples were verified by multiplex PCR assay (Supplementary Table S6) to exclude cross-contamination.
All of our positive samples were verified with sequencing.
Sequences from several samples were verified by extraction and amplification at the Griffith University Ancient DNA Facility, Nathan, Australia.
All samples were verified as Caucasian descendants.
Positive samples were verified by using neutralization test.
All the mutations detected in such samples were verified in the original nonamplified samples.
Before analysis, the validity of the study sample was verified.
Two SM-CWD brain samples were inoculated into squirrel monkeys and cynomolgus macaques to verify that SM-CWD was infectious, test for further adaptation, and to see if SM-CWD was infectious to a broader range of nonhuman primates.
Rat brain samples were homogenized in the same buffer used for the human brain samples.
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