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The ΔCt from the reference brain samples was subtracted from the ΔCt obtained from the liver samples.
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In all cases, the signal from background samples was subtracted from the sample fluorescence.
Fluorescence from IgG precipitated samples was subtracted from fluorescence from samples.
Average fluorescence of TSA treated samples was subtracted from the average of untreated corresponding samples.
The absorbance of the blank samples was subtracted from each sample absorbance.
Mean fluorescence intensities (MFI) of unstained samples were subtracted from the stained samples.
Background spectra of buffer samples were subtracted from the respective protein spectra.
Finally, the normalized value of untreated sample was subtracted from that of inorganic phosphate (Pi -treated samPi -treatedulate the change in calcium deposample.
The absorbance for each sample was subtracted from the blank and plotted.
The autofluorescence in each sample was subtracted.
The percentage positivity in the unstimulated control sample was subtracted from the stimulated samples.
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