Sentence examples for brain samples was performed from inspiring English sources
The qRT-PCR analysis of human brain samples was performed using our established methods [55].
Proteinase K digestion of brain samples was performed as described [27].
Western blot analysis from adult brain samples was performed as described by Porta et al. with minor modifications [5].
Storage and biochemical analysis of human brain samples was performed with consent from relatives and with approval from the Local Research Ethics Committee of the Institute of Neurology/National Hospital for Neurology and Neurosurgery (London, U.K).
ChIP from brain samples was performed using 50 70 mg of tissue homogenized using a tissue grinder after crosslinking and quenching, and following the same procedure used for fibroblasts with the exception that samples were incubated overnight at 4 °C with 2 μg of anti- pan H3 anti- pan H3Millipore), andwell as anti-H4K9mEMD H3K27Millipore9me3, H4K20me3 (Diasenode).
Measurements of DA and 5HTmetabolites in collected brain samples were performed by HPLC with electrochemical detection (ALEXYS LC-EC system, Antec Leyden BV, Netherlands) equipped with a reverse-phase column (3 μm particles, ALB-215 C18, 1 × 150 mm, Antec) at a flow rate of 200 μl/min and electrochemically detected by a 0.7 mm glass carbon electrode (Antec; VT-03).
Lipidomic analyses of brain samples were performed with a microfluidics ionKey/MS system composed of an ACQUITY UPLC M-Class, the ionKey source, and an iKey CSH C18 130 Å, 1.7 μm particle size, 150 μm × 100 mm column (Waters Corporation, Milford, Massachusetts, USA) coupled to a Synapt G2-Si (Waters Corporation, Manchester, UK).
Chromatography of brain microdialysate samples was performed on a Beckman Coulter™ Ultrasphere® 5 μm C-18 column (2 mm I.D. × 250 mm, Alltech) at a constant temperature of 30°C.
