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DNA from brain samples was obtained using QIAamp DNA Mini Kit (QIAGEN).
A selection of normal brain samples was obtained from BrainNet Europe/Barcelona Brain Bank and normal kidney samples were obtained from XBTC tissue bank.
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The brain samples were obtained from The Netherlands Brain Bank, Netherlands Institute for Neuroscience, Amsterdam (open access: www.brainbank.nl).nl
Brain samples were obtained at different intervals (0, 24, 48 and 96 h postmortem).
Then, brain samples were obtained at 3 different postmortem time intervals; after 24 h, 48 h and 96 h (6 rats for each interval).
Sporadic CJD brain samples were obtained from confirmed sCJD cases [31].
Frozen brain samples were obtained from the Netherlands Brain Bank (Amsterdam, the Netherlands) and the UK Multiple sclerosis tissue bank (London, UK).
In brief, brain samples were obtained at the Allegheny County Medical Examiner's Office (Pittsburgh) after written consent from next-of-kin.
AD and control brain samples were obtained from the tissue bank of the Swiss National Reference Centre of Prion Diseases (Zürich, Switzerland).
Twelve brain samples were obtained from four deceased patients who suffered an ICH including perihematomal tissue (PH) and the corresponding contralateral white (CW) and grey (CG) matter.
The autopsied brain samples were obtained from Research Resource Network (RRN), Japan.
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