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Filtering of genes abundantly expressed in hypopharyngeal glands (a potential source of tissue contamination in brain samples) was done as in [8].
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Samples were done in triplicate.
All samples were done in triplicates.
All the samples were done in triplicate.
In each brain, cells were counted from 3 independent slides, and sampling was done from the entire anterior posterior axis of the areas analyzed.
Sampling is done purposively.
Possible explanations include the different rat strains used, slight differences in the age at time of sacrifice, and slight differences in the dissection of the dorsal brain sample, which was done by separate individuals in the two trials.
Proteinase K digestion of brain samples was performed as described [27].
The brain samples were deproteinized by acetonitrile with IS.
For each study, several brain samples are needed from each group--diseased and healthy.
Embryonic brain samples were fixed overnight in 4% paraformaldehyde (PFA).
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