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Groups of mice were killed at 5 and 30 min p.i. Radioactivity of weighted brain samples was measured in a γ-counter (Wallac 1480-011 Automatic Gamma Counter, PerkinElmer, Waltham, MA, USA).
The qRT-PCR analysis of human brain samples was performed using our established methods [55].
Proteinase K digestion of brain samples was performed as described [27].
Western blot analysis from adult brain samples was performed as described by Porta et al. with minor modifications [5].
Protein concentration in boar sperm and boar brain samples was determined and standardized for all experiments according to the Lowry method [49].
Filtering of genes abundantly expressed in hypopharyngeal glands (a potential source of tissue contamination in brain samples) was done as in [8].
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The homogenized brain samples were imaged under 60x magnification.
Brain samples were processed as described in detail before51.
Brain samples were serially sectioned at the striatal region.
Rat brain samples were homogenized in the same buffer used for the human brain samples.
The brain samples were obtained from The Netherlands Brain Bank, Netherlands Institute for Neuroscience, Amsterdam (open access: www.brainbank.nl).nl
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