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The known miRNAs (344 precursors, 247 mature miRBase Release 19) comprised a wide range of the mapped reads (16 62 %), with the brain samples having the maximum amount (38 62 %) and with the ovary and testis having the minimum amount (6 8 %).
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Studies using postmortem human brain samples have demonstrated alterations in the levels of α7 nAChRs in the brains of patients with schizophrenia [16], [17] and Alzheimer's disease [18] [20].
Notably, one of the male brain samples had an extreme profile compared to the other male or female samples.
However, Ernst et al. [ 44] reported that the pH of brain samples has no significant effect on DNA methylation status.
In order to address this issue a number of potential confounding factors and their effect on transcript abundances in the post-mortem brain samples have been intensively studied [ 15- 22].
Although data from human postmortem brain samples has shown less global DNA methylation in the brains of alcoholics relative to controls [ 67], we found no correlation between methylation and alcohol use in the sample as a whole when controlling for effects of the other factors.
Targeted studies in mice looking at the patterns of histone modification at this imprinted locus in murine embryonic brain samples have identified enrichment of H3K4me2 and H3Ac modifications along the paternal chromosome, with H4K20me3-, H4K20me3-, H3K36me3-,and H3K79me3-modified nuclenrichedenriched along the silenced maternal copy [ 43].
Non-neoplastic brain samples had mean average methylation of 3.2±2.89 s.d.
PSP brain samples had significantly higher levels of tau oligomers, total tau, and phosphorylated tau.
Noticeably, AD brain samples had higher density bands of these two truncated forms compared to control samples.
Analyses of AD brain samples have demonstrated a higher burden of oligomeric Aß in APOE4 carriers with increased amyloid plaque-associated synaptic loss.
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