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Their study involved looking at large, intact brain samples from mice, as well as monitoring live mouse brains.
Examination of whole brain samples from mice infected with Brazil strain showed reduced Cx43 immunostaining [ 50].
We next studied brain samples from mice injected with the Mokola-miR124T lentiviral vector, allowing for specific astrocytic targeting.
RNA was isolated from the brain samples from mice stored in RNA later by spinning down and removing the RNA later from the tissue and then isolating RNA using the RNA-Bee protocol described above.
We further analyzed whether the density of linker histone H1 was altered in the RTT patient samples, as previously suggested in brain samples from mice with RTT-like phenotype.
Analyses of brain samples from mice subjected to MWM tests confirmed robust preservation of hippocampal neurons in mice, which have much improved learning and memory retention after NPS2143 treatment (Fig. 4E, TGI-NPS vs. TGI-DMSO).
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After conducting maze tests, the neuroscientist Fred H. Gage and his colleagues examined brain samples from the mice.
Brain samples from C57 BL/6 mice were isolated from anesthetized and perfused animals[63].
Applying real-time quantitative PCR, the mRNA expression of the presynaptic marker protein Synaptophysin (Syp) as well as the postsynaptic markers Disk large homolog 4 (Dlgh4) and Drebrin (Dbn1) were analyzed in brain samples from ARTE10 mice (Fig. 5, Fig. S9).
Immunohistochemistry confirmed MT overexpression at the protein level in brain samples from GLD mice and suggested that MTs are expressed predominantly by reactive astrocytes (see Supplementary Fig S3B, C).
On one hand, Kriaucionis et al. [37] detected higher Gtl2 levels in whole brain samples from late symptomatic mice, but on the other, another report [31] could not confirm differences in Gtl2 expression between WT and KO in cerebellum or forebrain using qRT-PCR.
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