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Cells were isolated from blood and brain samples for flow cytometric analysis to identify GFP+ cells in the tissues.
Dr. Kari Stefansson, a neurologist and DeCode's chief executive, noted it was extremely hard to get enough schizophrenic brain samples for good statistical analysis but, given that constraint, the new finding was promising and "remarkable if true".
However Bauer et al. (2003), demonstrated in their study a significant correlation between RNA degradation and PMI in stored refrigerated human brain samples for up to 5 days.
We consistently observed small numbers of TUNEL-positive cells throughout the brain samples for all ages.
Such a large-scale genomic investigation is costly and time-consuming; hence we selected a small panel of well-characterized brain samples for DNA methylation profiling.
To prepare brain samples for immunostaining, Nissl and Fluoro-Jade B staining, and TUNEL labeling, mice were euthanized with carbon dioxide and perfused with 4% paraformaldehyde in phosphate-buffered saline (PBS).
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An arbitrary significance level of 0.01 was chosen from the linear regression results to establish these 2 lists (this differed from the brain sample for which a threshold of 0.05 was set due to the number of genes exceeding significance in lymphocytes and a 2000 gene limit set by the DAVID web tool).
Therefore, we combined equal concentrations of olfactory bulb, telencephalon and diencephalon/mesencephalon as a single brain sample for each replicate.
Figure 4(A) shows the relative changes in mRNA prepared from whole-brain samples for these genes in each of the mouse strains used in the study.
Authors thank NNTC for providing brain samples used for this study and Dr. Ashley Schoell (Los Angeles) for facilitating that.
A list of the number of brain samples heterozygous for the mSNP for each candidate gene is provided in Additional file 2, Table S5.
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