In addition, rCBF values in predefined pain and control brain regions were obtained.
In total, samples from 6 different brain regions were obtained and each brain region was sampled from a total of 4 animals.
Three images of each of following brain regions were obtained: corpus callosum, cortex, ventral midbrain, dentate gyrus, hippocampus (CA1/CA2), and striatum.
From each subject, ten different brain regions were obtained: frontopolar cortex (Brodmann Area 10; BA10), Wernicke's area (BA22), anterior cingulate cortex (BA24), dorsolateral prefrontal cortex (BA46), insular cortex, hippocampus, amygdala, posterior putamen, cerebellum, and brainstem raphe nuclei.
From each subject, ten brain regions were obtained to provide a diverse set of brain regions: frontopolar cortex (Brodmann Area 10; BA10), Wernicke's area (BA22), anterior cingulate cortex (BA24), dorsolateral prefrontal cortex (BA46), insular cortex, hippocampus, amygdala, posterior putamen, cerebellum, and brainstem raphe nuclei.
It remained possible that brain regions other than frontal cortex might contain more readily CDI-detectable PrPSc in cases 1, 2 and 4. Samples of additional brain regions were obtained for case 2 and these were analysed by CDI without prior PK treatment or with mild PK treatment (2.5 μg/ml).
Using Lassen plot, a linear fitting to the data points from seven brain regions was obtained for the dose of 1.5 and 4.5 mg/kg of SSR504734 (Figure 9A,B).
In some of the inoculated cattle, PrPres profile was different based on what brain region was obtained [37].
For each sample, a representative time series of each brain region was obtained by averaging the fMRI time series over all voxels in the region.
The ADC and percent ADC values (the ADC percentage as compared to the control data from the corresponding region) were obtained in various regions of the brains.
A similar outcome, of no significant differences between experimental groups, in the three investigated brain regions, was also obtained when we calculated percentages of [H]-thymidine+ neurons = 0.8405, P = 0.448; HVC: F 2,17) = 0.0692, P = 0.933; HC: F 2,17) = 0.9245, P = 0.415).
