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Fig. 2 Mean decay-corrected TACs of five brain regions after intravenous injection of [11C]CB184 into typical human subjects (a).

It could be inferred from those studies that (1) intranasal instillation might be the most effective routes for TiO2 NPs transported to the brain and (2) Ti content could be undetectable in the brain regions after intravenous injection.

However, the distribution of radioactivity in the brain regions after intravenous administration of [76Br]SSR180711 was not altered by pretreatment with the selective α4β2 nAChR agonist A85380 (1.0 mg/kg, i.v., 30 min)[29], [29](Figures 2, 3 and 4B).

The distribution of radioactivity in the brain regions after intravenous administration of [11C]CHIBA-1001 was blocked by pretreatment with the selective α7 nAChR agonist SSR180711 (5.0 mg/kg).

Uptake of radioactivity in the brain regions after intravenous administration of [76Br]SSR180711 was significantly decreased by pretreatment with the α7 nAChR agonist SSR180711 (5.0 mg/kg, i.v., 30 min)(Figures 2 4).

Furthermore, a preliminary study indicated that the uptake of radioactivity in the brain regions after intravenous administration of [11C]CHIBA-1001 walsolso decreased by pretreatment with another α7 nAChR agonist A844606 (5.0 mg/kg, i.v., 30 min before) [30] (Supplemental Figure S1).

In contrast, eEF2 phosphorylation was elevated in all brain regions after sleep deprivation.

PACAP-receptors are found in the entire brain and one study reported uptake of I-PACAP38 in all brain regions after intranasal administration [17].

We have reported that Escherichia coli K-12 colonizes hypoxic and necrotic tumor regions after intravenous injection into tumor-bearing mice.

Also, the change of IL-1β expression in brain regions was examined after behavior test.

PET images were smoothed with a Gaussian filter of 4 mm in FWHM. Figure 2a shows the mean time activity curves (TACs) in five brain regions of typical subjects (n = 4) after intravenous injection of [11C]CB184.