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However, we worked in vivo, while Rosay et al., worked on an in-vitro brain preparation.
The NALCN channel was first cloned from rat brain preparation [41].
We used an in vitro model of the neurovascular unit and the guinea pig whole brain preparation to analyze with immunohistochemical methods the BBB properties and neurovascular integrity.
Interestingly, preliminary observations demonstrate that other cell types, such as activated lymphocytes and polymorphonucleates that adhere to inflamed endothelium in the isolated brain preparation, tend to avoid the ischemic core in our experimental ischemic conditions.
However, we also detected disruptions of ZO-1 after MCAO in the cortical MCA territory of the guinea pig whole brain preparation which resembles more closely the in vivo situation.
MCA occlusion for 30 minutes in the in vitro isolated guinea pig brain preparation was followed by arterial perfusion with 1×106 NCs charged with a green fluorescent dye, either immediately or 60 minutes after reperfusion onset.
In support of this hypothesis it should be noted that herein it is shown that the addition of c-Fos both to a non-pathological brain preparation obtained from a GM-patient as well as to the tumor sample stripped of associated proteins (Fig. 2) results in an activated rate of phospholipid synthesis.
Whole brain preparation and electrophysiology.
Imaging experiments were performed using a naked brain preparation.
For brain preparation, mice were anaesthetized with pentobarbital (40 mg/kg, i.p).
(B ) An example of the swim motor pattern recorded from an isolated brain preparation.
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