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Brain microvessels were obtained as described previously [18].
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Total RNA from isolated brain microvessels was obtained by lysing the surrounding basal lamina with proteinase K and then extracting total RNA using the RNeasy Fibrous Tissue Micro kit according to the manufacturer's instructions.
To explain Aβ enhanced clearance across the BBB, brain microvessels were isolated for analysis.
13 After removing the leptomeninges from the brain, microvessels were isolated out of the grey matter.
Brain microvessels were isolated as described previously (Seelbach et al. 2007).
All steps in the isolation of brain microvessels were carried out at 4°C.
Brain microvessels were isolated according to the method of Yamakawa et al. [ 17].
In the present study, isolated brain microvessels were used to examine the expression of proteins that mediate angiogenesis and accurately corresponded to the observed alterations in the brain microvessels; significantly lower VEGF expression was observed in the aging rats.
Brain microvessels were isolated from naïve mice and mice infected 7 days earlier with PbA, PbNK65 or Py17X.
Isolated brain microvessels were homogenized in 500 μL of ice-cold RIPA buffer containing a protease inhibitor cocktail (Sigma, USA).
Isolated brain microvessels were homogenized in lysis buffer (Sigma, St . Louis MO, USA) containing Complete® protease inhibitor (Roche, Mannheim, Germany).
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