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Isolated brain microvessels were also free from red blood cells, other contaminating cells such as microglia, and cell debris [ 27].
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Consistently, the endothelial localization of CXCL1 and CX3CL1 in brain microvessels was also observed 4 h post-CLP (Supplementary information, Figure S4A and S4B).
An increased number of microvessels were also observed on the site of EPC injection [ 49].
Brain microvessels were obtained as described previously [18].
To explain Aβ enhanced clearance across the BBB, brain microvessels were isolated for analysis.
13 After removing the leptomeninges from the brain, microvessels were isolated out of the grey matter.
Brain microvessels were isolated as described previously (Seelbach et al. 2007).
All steps in the isolation of brain microvessels were carried out at 4°C.
Brain microvessels were isolated according to the method of Yamakawa et al. [ 17].
In the present study, isolated brain microvessels were used to examine the expression of proteins that mediate angiogenesis and accurately corresponded to the observed alterations in the brain microvessels; significantly lower VEGF expression was observed in the aging rats.
Brain microvessels were isolated from naïve mice and mice infected 7 days earlier with PbA, PbNK65 or Py17X.
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