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There are also developmental changes of FGF19 gene expression in brain microvessels collected from 2-month-old to 8-year-old humans [ 28].
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The scrapie inocula were prepared from brain tissues collected from terminally sick mice.
Brains were collected from wild caught, sexually mature individuals.
Lungs, liver, brain, and serum were collected from these animals.
Assessment of tight junction protein levels in brain microvessels isolated from C57BL/6 and C57BL/6 Prf1−/− mice demonstrated that perforin was necessary for alteration of BBB tight junction protein levels in C57BL/6 mice.
qPCR profiling of brain microvessels isolated from conditional mutants showed alterations in pericyte-expressed proteoglycans while other genes previously implicated in pericyte endothelial cell interactions were unchanged.
However, using the TCR-transduced reporter cell line, we determined that this peptide-MHC complex was presented on brain microvessels isolated from PbA-infected mice, but not from mice infected with the non-ECM-causing parasites.
When brain microvessels are isolated from adult mammalian brain and brain endothelial cells are cultured from these vessel fragments, they retain many key features of the BBB phe-notype.
In fact, conditioned medium collected from microvessels of AD patients has been shown to kill neurons in vitro, pointing to selective neurotoxic factors derived from brain capillary endothelial cells (Grammas et al., 1999).
Cyp1a1 transcripts were not quantified in brain microvessels from control- and Δ-THC-treated rats.
Brain microvessels were isolated from naïve mice and mice infected 7 days earlier with PbA, PbNK65 or Py17X.
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