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Brain microdialysates from each patient were individually analysed for a pre-dose monitoring period typically lasting 4 8 h to establish a baseline.
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An unknown interference of ACh, which was observed in brain microdialysates from many studies, was well separated from ACh to ensure the accuracy of the measurement.
A preliminary report illustrating VGB concentrations vs. time in brain microdialysates from three patients from this cohort did not include PK calculations 14.
The brain microdialysates from TBI patients in the present study showed a trend for increase in GABA concentration from 0.27 to 0.51 μmol l−1 in 24 h, after two VGB doses in four patients (P = 0.06).
The maximum VGB concentration in brain microdialysates after the first dose (Cmax,br1), shown in Figure 2 for each patient, had a median (IQR) of 2.41 (2.03–5.94) μmol l−1, with a full range of 0.74 28.3 μmol l−1.
This study reports an Fe3+-functionalized carbon quantum dots (Fe3+-functionalized CQDs) for the highly sensitive and selective detection of ascorbic acid (AA) in rat brain microdialysates based on the specific redox reaction between iron III) ions and AA.
In brain microdialysates, VGB median (IQR) Tmax,br1 was 5.1 (4.3 7.4) h.
Also in brain microdialysates, we analysed concentrations of GABA, as a downstream marker.
Microdialysate collection vials were changed every 30 min for a period of 5 h and stored at −80°C for subsequent analysis by HPLC using the same method as for brain microdialysates (see below).
In brain microdialysates, the working lower limit for VGB was ∼0.4 pmol injected (corresponding to 1.2 μmol l−1 in the microdialysate) due to an unknown substance with a similar retention time to VGB.
In the brain DA-Conjugates seem to play only minor roles as in rat brain microdialysates DOPAC and HVA are the main metabolites by far [ 135].
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