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Brain microdialysate samples were acidified with nitric acid immediately after collection by addition of 1 μl HNO3 (conc).
TMZ concentrations in plasma and normal brain microdialysate samples were determined using a validated reversed-phase high-performance liquid chromatography method with ultraviolet detection.
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Chromatography of brain microdialysate samples was performed on a Beckman Coulter™ Ultrasphere® 5 μm C-18 column (2 mm I.D. × 250 mm, Alltech) at a constant temperature of 30°C.
The microdialysate samples were analyzed at the Painomics Laboratory, Rehabilitation Medicine, Department of Medical and Health Sciences, Linköping University, Sweden.
The microdialysate samples were collected every 15 min after an hour of equilibration period.
Microdialysate samples were analysed using a mobile photometric, enzyme-kinetic analyser (CMA Iscus Flex) for glycerol concentration.
All the microdialysate samples were then frozen (−20°C) for later analyses.
Microdialysate samples were collected every 4 hours and analyzed with a colorimetric method for glucose, glycerol, lactate and pyruvate.
MD catheters were left in-situ for at least one hour for stabilization before microdialysate samples were collected.
Microdialysis catheters were left in-situ for at least one hour for stabilization before microdialysate samples were collected.
Serial plasma and microdialysate samples were collected over 24 h, and temozolomide concentrations were measured using tandem mass spectrometry.
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