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Each tissue microarray also contained samples of non-neoplastic gray and white brain matter (obtained from epileptigenic brain), infiltrating ductal carcinoma breast tissue, six GBM xenograft cell lines, and U87 and U251 conventional glioma cell lines.
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The non-tumor brain samples consisted of histologically-normal temporal lobe white matter obtained from patients undergoing surgery for epilepsy.
Postmortem white matter brain tissue from 19 MS patients and 9 brain donors without brain disease was obtained from the Netherlands Brain Bank (http://www.brainbank.nl).nl
White matter brain samples were obtained from seven MS patients (average age: 61, 57% male, 57% secondary progressive multiple sclerosis SPMS, average disease duration of 37 years) in collaboration with the Netherlands Brain Bank, Amsterdam, The Netherlands (coordinator Dr. Huitinga).
Normal brain samples, which included cortex and white matter, were obtained from autopsy cases without any evidences of brain tumor or other brain disease.
The human brain tissue was obtained from The Netherlands Brain Bank, Netherlands Institute for Neuroscience, Amsterdam.
Human brain tissues were obtained from The Netherlands Brain Bank, Netherlands Institute for Neuroscience, Amsterdam.
Brain sections obtained from perfused brains were mounted on slides and were stained with Cresyl Violet.
Brain tissue was obtained from the Quebec Suicide Brain Bank.
However, we did not consider this staining property to be a reliable marker of a neuronal abnormality, since many neurons in neocortical and hippocampal gray matter in samples of brains obtained from temporal lobe epilepsy and other non-epilepsy surgeries revealed high levels of p-S6 immunoreactivity (unpublished data).
The colors of brain structure were obtained from the ABA, where similar colors represent related brain structures.
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