Sentence examples for brain lysates from each from inspiring English sources

ELISA assays were performed for TNFα and IL-6 on brain lysates from each of the mice used in the study.

The chaperone expression in each genotype was confirmed by western-blotting two sets of brain lysates from 7-month old mice for αB-crystallin, and Hsp27 (Figure 2B).

Brain lysates from CPD1 null mice were subjected to western blot analysis.

To confirm disruption of GASP-1 expression, brain lysates from WT, heterozygous (HET) and KO mice were immunoblotted for GASP-1 (Fig. 1D).

Brain lysates from wild type (WT), heterozygous (HET) and KO mice were separated on a 4 20% gradient Tris·HCl precast gel (Invitrogen, Carlsbad, CA) and transferred to 0.22 µm nitrocellulose at 200 mA for 4 h.

Consistent with a role of PS in the proteolysis of neurexins, we found a significant accumulation of neurexin-CTFs in brain lysates from PS1−/− embryos and PS cDKO adult mice compared with control littermate animals (Fig. 4A).

The interaction is specific since GST does not bring down APP and a molecule reacting with the anti-APP antibody is not isolated by GST-Mint1 when brain lysates from APP KO mice are used (Fig. 3).

To address the possibility that this increased aggregation of 2UIM ataxin-3 reflects transient overexpression in nonneuronal cells, we compared levels of SDS-insoluble ataxin-3 aggregates in whole brain lysates from aged MJD84.2 mice, which express primarily expanded Q84 3UIM ataxin-3 transprotein, and Q71B mice, which only express expanded Q71 2UIM ataxin-3 transprotein (Figure 4C and 4D).

We performed Ack1/TrkB immunoprecipitations in brain lysates from embryonic E16 brains.

We then performed subcellular fractionation of brain lysates from 3 6 months old WT mice.

Similarly, Nrp1 protein was expressed in detergent-soluble brain lysates from control and β8 −/− mutant embryos (Fig. S4D).