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CCS values were used as additional molecular descriptors to identify brain lipids using a variety of traditional lipidomic approaches.
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Body and liver weight did not show statistical differences between the four groups; however, cumulative alcohol consumption was 21% higher (unpaired t test, p-value = 0.0060) in the JUV group compared to the ADU group Total lipids were extracted from brain homogenate using a modified Folch extraction method.
In vivo brain imaging using a portabler3.
Peroxidase catalyzed oxidation of lipids using hydrogen peroxide as an electron acceptor.
RNA from LV-injected brains was extracted using a RNeasy Lipid Tissue kit (Qiagen).
The residual lipids extracted from the paraffin embedded brain samples were analyzed using a procedure recently described [ 13].
Vacuole lipid levels were measured using a lipid phosphorus assay.
We evaluated the causal relationship between plasma lipids and DR using a Mendelian randomization approach.
Kyrklund T. Two procedures to remove polar contaminants from a crude brain lipid extract by using prepacked reversed-phase columns.
Next, qualitative MILDI-MS imaging of CER and SM at three coronal brain levels were obtained and imaged using a new technique of Silver nanoparticulate (AgNP) matrix implantation which enables simultaneous identification of most brain lipid classes, except gangliosides.
North, P. & Fleischer, S. Alteration of synaptic membrane cholesterol/phospholipid ratio using a lipid transfer protein.
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