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Another limitation is the fact that brain images were obtained using different imaging protocols.
High-resolution brain images were obtained from a water-filled phantom and from a healthy volunteer brain.
Axial, coronal, and sagittal brain images were obtained.
Brain images were obtained from 13 transverse slices (5 mm slice thickness, 2.5 mm slice interval) on the base of the anterior commissure-posterior commissure line or orbitomeatal line.
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The brain structural images were obtained from 32 normal controls (NC) and 44 MOH patients on 3.0 T MR system.
The brain structural images were obtained from 18 normal controls (NC), 18 EM patients and 16 chronic migraine (CM) on 3.0 T MR system.
Secondly, the brain structural images were obtained bya three-dimensional T1-weighted fast spoiled gradient recalled echo (3D T1-FSPGR) sequence generating 180 contiguous axial slices [TR (repetition time) = 6.3 ms, TE (echo time) = 2.8 ms, flip angle = 15o, FOV (field of view) = 25.6 cm × 25.6 cm, Matrix = 256 × 256, NEX (number of acquisition) = 1].
To investigate individual brain anatomy and to exclude possible brain lesions, structural MR images were obtained from every subject.
To identify brain regions, magnetic resonance (MR) images were obtained using a 3-T GE Signa device (GE Healthcare, Milwaukee, WI, USA).
Standard brain MRI sequences, including sagittal images, were obtained in all patients and were interpreted by consultant neuroradiologists at our respective clinical neurosciences centres.
Brain three-dimensional T1-weighted structural images were obtained from 44 MOH patients and 32 normal controls (NC).
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