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Twenty-µm cryosections of the brain hemispheres were cut and serial hematoxylin and eosin staining was performed to identify the brain regions with the structures of interest according to a rat brain atlas [41].
Briefly, brain hemispheres were cut in a frontal plane into 30 μm-thick sections, and serial sections were photographed with a digital camera at 0.3 mm-intervals.
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The two cerebral hemispheres were cut into 2 mm-thick serial sections using a brain mold and snap-frozen.
Then hemispheres were cut using a freezing microtome.
Coronal sections from both hemispheres were cut at 40 µm thickness using a freezing microtome.
Hemispheres were cut in the coronal plane in 40 μm thick sections and cryo-protected.
Coronal 10 μm sections of the entire hemisphere were cut for subsequent staining.
(C) Ex vivo rat brain was dissected into two hemispheres--the left hemisphere was cut into 40-μm thick sagittal brain sections and were scanned to reveal brain areas.
Here, paraffin-embedded tissue microarrays (human or mouse) or sagittal brain hemispheres (mouse) were cut into 5 μm sections, placed on glass slides and stained as previously described (72).
Formalin-fixed brains were cut and divided on the midline; 1 hemisphere was cut in the sagittal plane; the other was cut coronally at the anterior basal ganglia, the middle of the thalamus, and the brainstem with cerebellum.
Brains were harvested immediately after perfusion, hemispheres were separated, and one hemisphere was fixed in 10% neutral buffered formalin (for immunohistochemical evaluation and imaging) while the other hemisphere was cut into 1 cm coronal slices and rapidly frozen (for biodistribution quantification).
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