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One brain hemisphere was fixed in 10%% buffered formalin for more than several months.
From all animals one brain hemisphere was fixed in 4 % paraformaldehyde for 24 h for histological examination.
The brain hemisphere was fixed with 10% buffered formalin for an average of 408 days in the control group and 905 days in the affected group.
One brain hemisphere was fixed in 10% buffered formalin for 1.5 to several months and then dissected into 1 cm thick slabs.
The brain hemisphere was fixed with 10% buffered formalin for an average of 408 days in the control group (range 52 1,819 days) and 905 days (range 75 4,560 days) in the autistic cohort.
Brains were collected and the right brain hemisphere was fixed in 10% buffered neutral formalin (BNF) for 3 4 weeks.
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Brains were longitudinally cut, for each brain one hemisphere was fixed in buffered formalin and the other was snap frozen on liquid nitrogen.
Left ACC of rats' brain in each group used for IF was fixed and post-fixed in 4% paraformaldehyde (pH 7.3), dehydrated in 15 30% gradient sucrose and frozen at −80°C.
Will the AMT be fixed?
For the oligo-based purification of RNA-associated proteins and in vitro biotinylated RNA pull-down of proteins, one hemisphere of a WT adult C57BL/6 brain was fixed in 3.7% formaldehyde/1× phosphate buffered saline (PBS) and homogenized.
One hemisphere of the brain was fixed in 10%% phosphate-buffered formalin, and the other hemisphere was coronally sectioned and then frozen at −80 °C until use.> -wrap-foot> Aβ42: -, negative; +, small aggregates of Aβ42 were observed in the parietal and temporal cortices on FA-pretreated sections, and intracellular aggregates of Aβ 42 were observed in the cytoplasm of hippocampal pyramidal cells.
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