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One optic field covering ≥100 cells per central brain hemisphere from each brain was included; samples were collected from two to three independent aging crosses.
Quantification was done manually using Zeiss software for 3D imaging; only well distinguishable individual TUNEL-positive cells (≥2 μm) were analyzed; one central brain hemisphere from each brain was included; samples were collected from three independent aging crosses; six to eight heads per condition were analyzed.
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Clones for sequencing were drawn first from two normalized libraries (designated SB01 and SB02) representing zebra finch brain RNA from both sexes at multiple ages.
Every run contained a negative control for background subtraction (water instead of RNA) and a positive control (total human brain RNA from Ambion, provided by Luminex Inc).
Every run contained a negative control for background subtraction (water instead of RNA) and a positive control (total human brain RNA from Ambion, provided by Luminex).
This is the first joint study of whole-genome mRNA and miRNA profiling using individual human brain RNA from autopsies of HAD and HIV non-dementia patients.
These functions were performed sequentially for each brain MRI slice from front to back.
9) You know, maybe Moran's guts-over-brains viewpoint on baseball is what prompted the infamous "Get A BRAIN! MORANS" sign from this St Louis Cardinals fan.
Total RNA was extracted from a brain hemisphere using RNA-Bee (Tel-test Inc., Friendswood, USA).
When a mouse was harvested, one hemisphere from each mouse brain was frozen on dry-ice and kept at -80 °C, and these tissues were used for ELISA analysis.
MSA-mNEPv in mouse brain was extracted as follows: one hemisphere from each mouse was transferred to homogenizing tubes containing 1.4 mm zirconium oxide beads (CK14, Bertin Technologies) and PBS containing protease inhibitor cocktail (Complete™, Roche diagnostics) at a ratio of 3 1 (3 µl of PBS: 1 mg brain).
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