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Nevertheless, all four species performed comparably on the A. mellifera cDNA microarrays and maintained consistent patterns of brain gene expression differences.
However, we did not use whole bodies for two reasons: 1) our primary interest in brains and behavioral castes, and 2) the fact that brain gene expression differences in social insects can be subtle [ 57], meaning that high levels of expression from the fat body and other tissues could have drowned out expression patterns from the brain.
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In the brain, most gene expression differences between the sexes are not expected to be that high [ 44].
Investigations of expression differences of brain gene expression between humans and nonhuman primates have been done for several brain regions using microarrays (Enard et al. 2002; Caceres et al. 2003; Khaitovich et al. 2004, 2005; Uddin et al. 2004; Somel et al. 2009).
Reasoning that genes controlling differences in dispersal and host-selection behavior are likely to be expressed in the brain, we therefore searched for gene expression differences in heads of female beetles using a cDNA-based microarray.
To determine if the four brain regions examined have unique gene expression differences, we examined the overlap of genes with differential expression across the four brain regions.
PC 4 reflected a pattern in the data that indicates that a component of the variance in brain gene expression is attributable solely to differences between A. dorsata and the other species.
Furthermore, the significant differences in brain gene expression demonstrate that high vs low responding bees are indeed in different physiological states, either due to genetic factors, developmental cues that results in fixed physiological differences into adulthood, or environmental differences in QMP exposure.
The resulting workers within each patriline shared, on average, 75% of their genome, which standardized the genetic background against which differences in brain gene expression patterns were assessed.
Here, we use brain gene expression age-series to investigate possible differences in aging rate between sexes.
1772 genes showed significant differences in brain gene expression between foragers and one-day-olds (ANOVA, F-test, p < 0.001) in any species.
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