Exact(6)
What's more, even if the researchers succeed, they will only produce a static picture of a brain frozen in time, rather than something that shows how a brain responds to different types of stimuli.
Immunochemical analysis was performed as on 12 μm brain frozen sections.
Brain frozen sections were stained with H&E and Ki-67 (Thermo/lab Vision Cat# RB-90-43 RB-90-43 RB-90-43
Approximately 50 mg of mice brain (frozen at −80°C) was plunged into 1500 μL of 50% acetonitrile/Milli-Q water containing internal standards at 0°C to inactivate enzymes.
Rat brain frozen sections were prepared and processed as described and were probed with digoxigenin-labeled DR6 antisense probe (5′-TAATACGACTCACTATAGGGGCTGGTGGGTAAGTTGTGGT-3′) and sense RNA probe (5′-ATTTAGGTGACACTATAGAACTCGCGGTACCTTCTCTGAC-3′).
Unfortunately, even though the DASL-based assay performed better than the IVT-based assay in limited brain frozen tissues, our sample sizes were too small to make conclusive arguments about the relative efficacy of DASL- and IVT-based platforms on RNA extracted from frozen and formalin fixed tissues.
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