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Consistent with previous reports, Zip7 was detected in whole murine brain fractions containing the Golgi marker, Golgi 97 (Huang et al., 2005).
Zinc, copper and cobalt were elevated in the CLN6 brain fractions containing the lysosomal marker, lysozyme, the early endosome marker, Rab5, and the Golgi marker, Golgi 97 (Fig. 7A,B,D).
At the terminal stage of the disease, we measured the levels of AChE transcripts, protein and enzymatic activity in cerebral cortex-enriched homogenates and in the brain fractions containing the remaining structures from each infected mouse (n = 5) and age-matched mock-infected controls (n = 5).
Similar(57)
The fractions containing the carbohydrates were pooled and freeze-dried.
Fractions containing the purified biomarker were vacuum dried.
Eluate fractions containing the Prod1 monomer were pooled and concentrated.
Fractions containing the protein were collected together.
The fractions containing the product were pooled and evaporated.
The fractions containing the desired product were collected and lyophilized.
The fractions containing the eEF2K complex were pooled.
The fractions containing the product were collected and lyophilized.
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