Sentence examples for brain extracts were prepared from inspiring English sources

Newborn rat brain extracts were prepared as reported previously [ 24].

Mouse and human brain extracts were prepared and western blot analyses performed as described previously (Teismann et al., 2003).

Brain extracts were prepared from end-stage (5 5.5 months) P301S tau mice and age-matched WT controls, as previously described [ 9].

Adult mouse brain extracts were prepared by homogenization in lysis buffer (PBS, 0.5% Triton [vol/vol], protease inhibitor mixture [Roche]) followed by ultracentrifugation (100,000× g, 60 min, 4°C) to remove insoluble material.

Brain extracts were prepared from cerebral cortex tissue pooled from two adult WT and LRRK2 KO mice, or human G2019S LRRK2 transgenic and non-transgenic mice by homogenization in TEVP buffer (10 m m Tris HCl, pH 7.4, 5 m m NaF, 1 m m Na3VO4, 1 m m EDTA, 1 m m EGTA) containing 320 m m sucrose.

Mouse and human brain extracts were prepared by homogenizing tissue in ice-cold lysis buffer (10 mM Tris-HCl (pH 7.4), 0.8 M NaCl, 1 mM EGTA, 10% sucrose, 1 mM DTT) supplemented with protease inhibitor cocktail and phosphatase inhibitor cocktail.

Total brain extract was prepared from adult Sprague Dawley cerebella in a manner similar to the GST pull-down experiments.

Brain protein extracts were prepared following the methods of Pradel et al. [20] (see File S1 for protocol).

Brain protein extracts were prepared for the western blots.

Total protein extracts were prepared from brain tissue.

Immunoprecipitation with anti-SUMO-1 followed by immunoblotting with anti-lipin-1 revealed a prominent sumoylated form of lipin-1 in brain, but only when the tissue extracts were prepared in the presence of NEM (Fig. 4B).