Sentence examples for brain extracts were obtained from inspiring English sources
Porcine brain extracts were obtained from a local slaughterhouse (Louis Gad SA) and directly homogenized and processed for affinity chromatography or stored at −80°C prior to use.
In brief, brain extracts were obtained from non-transgenic and FLAG-LRRK2 mice (P30) by homogenizing 2 brains with a motor-driven homogenizer (speed 2.5, 12 strokes) in 3 ml buffer containing 0.32 M sucrose, 1 mM NaHCO3, 20 mM HEPEs/pH 7.4, 1 mM MgCl2, 0.25 mM CaCl2, EDTA-free protease inhibitor cocktail, 200 µg/mL PMSF, pepstatin 4 µg/mL and DNase I.
Brain protein extracts were obtained from frozen brain tissue and processed for Western blotting [22].
Nine-month-old knockout or control mice of the same age were sacrificed by cervical dislocation, liver and brain tissues were dissected and tissue extracts were obtained as described below.
PrPres extracts were obtained from animal brains by using Bio-Rad protocol (the TeSeE Western Blot kit, ref: 355 1169; Bio-Rad, Marnes-la-Coquette, France), which is used to confirm suspected TSE-positive samples and includes PK digestion and rapid protein precipitation steps.
For this purpose nuclear extracts were obtained from the central brain at different time points during extinction session.
