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Sarkosyl-insoluble FTLD-TDP extracts or age-matched non-pathological brain extracts were analyzed by immunoblot using an antibody specific for phosphorylation at Ser409/Ser410 and a C-terminus anti-TDP-43 antibody (Fig. 1b, left panel).
Both CSF and brain extracts were analyzed by blue native-PAGE.
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Total glutathione in mouse brain extracts was analyzed using a Total Glutathione Quantification Kit (Dojindo) on a Spectra Max 190 (Molecular Devices).
(A) Soluble extracts were analyzed on 12% denaturing SDS-PAGE.
Neutral lipid extracts were analyzed by GC/FID and peaks identified by GC/MS (Agilent, Santa Clara, CA, USA).
Three separate extracts were analyzed.
These extracts were analyzed by GC-MS.
Extracts were analyzed using gas chromatography-mass spectrometry (GC-MS).
Bacterial cell extracts were analyzed by Western blotting after SDS-PAGE.
The final extracts were analyzed by FIA-MS/MS.
The resulting extracts were analyzed by immunoblotting.
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