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The most common quality control issue encountered was suboptimal brain extraction using the BET tool.
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(2) The normalized T1 images were performed with brain extraction using FSL (v5.0) tool to delete the non-brain tissue, and obtained a good brain T1 images (Fig. 1c).
As a first step, a tensor model was fitted to the raw diffusion data (using the FDT) to create fractional anisotropy images, which were then brain-extracted using the brain extraction tool (BET) (Smith, 2002).
Initially non-infectious pork brain was mixed with German standard soil and the efficiency of PrPC extraction using the above mentioned buffers was tested.
First, the raw T1-weighted images were brain-extracted using the FSL default BET brain extraction process, which strips the skull and removes any nonbrain tissue from the image using the FAST4 tool.
Each subject's whole brain was extracted using the Oxford centre for functional MRI of the brain (FMRIB) software library (FSL) [24] brain extraction tool (BET) [25] and the corresponding grey matter probability maps were created using statistical parametric mapping (SPM5) software [26].
For each individual resting states scan, the following pre-statistics processing was applied: motion correction using FMRIB's Linear Image Registration Tool (Jenkinson et al., 2002); brain-extraction using BET (Smith, 2002); and high pass temporal filtering of 150 s.
The following pre-statistics processing was applied to each individual run: motion correction using FMRIB's Linear Image Registration Tool (MCFLIRT [ Jenkinson et al., 2002]); brain-extraction using BET (Smith, 2002); mean-based intensity normalization; high pass temporal filtering of 300 s; and spatial smoothing using a Gaussian kernel of FWHM (full width at half maximum) 5 mm.
Venous sinuses and CSF were extracted using the brain extraction tool (BET) v.2.1 (Smith, 2002) in FSL v.4.1 (www.fmrib.ox.ac.uk/fsl).ac.uk/fsl
In such instances it was necessary to re-run the brain extraction step using the "bias field correction and neck cleanup" option in BET.
Brain extraction was performed using the brain extraction tool in FSL (Smith 2002).
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