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We assessed brain expression for each candidate gene via Unigene's EST Profile database [ 20], then returned to the LCLs and used rt-QPCR to confirm differential expression in 6 genes randomly selected from the set.
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To seek patterns of human brain expression for the genes identified herein, we identified 846 human cDNA libraries constructed from brains with modest or no pathology in dbEST.
Of the 107 genes we identified as K-Channel genes in zebra finches, we found direct evidence of brain expression for 76 (Table 1).
The derived data set was used to calculate ratios of forager to day-old brain expression levels for every gene and each array.
We calculated gene expression ratios of forager to one-day-old brain expression levels for every gene and each replicate pair of arrays (data not shown; mean ratios available in an online repository [ 21]).
Allen Brain Atlas contained human brain expression data for six different donors with data on various brain areas for different donors.
There were species differences in brain expression profiles for functionally related groups of genes.
Species differences in brain expression profiles for functionally related groups of genes provide clues to the basis of behavioral variation in the genus.
Species differences in brain expression profiles for functionally related groups of genes provide possible clues to the basis of behavioral variation in the genus.
Fly lines used were elav-gal4 (C155) for neural and brain expression, rh1-gal4 for photoreceptor cell-specific expression, and gmr-gal4 for eye expression.
Digital gene expression (DGE) analysis of parasitized honey bee brains demonstrated that, despite the difference in developmental stage at which the bee is parasitized, Nosema and Varroa-infested bees shared more gene changes with each other than with honey bee brain expression gene sets for forager or nurse castes.
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