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In the present study we expanded the study of the interaction of Vsp1 and Vsp2 with brain endothelium using a variety of approaches, including: (1) metabolically labeled Vsp1 and Vsp2 expressing B. burgdorferi transformants; (2) radiolabeled sonicated Bt1 proteins; (3) lanthanide-labeled lipidated and non-lipidated Vsp1 and Vsp2.
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Here we studied the interaction between Vsp1 and Vsp2 with brain endothelium using cell association assays with radiolabeled recombinant Borrelia burgdorferi transformants displaying LVsp1 or LVsp2 in their surface, radiolabeled sonicated proteins from Bt1, and lanthanide-labeled purified LVsp1 and LVsp2 present alone or in combination in vitro and in vivo.
Here we showed an over-view of the cellular process altered in cytokine-activated brain endothelium using mRNA array analysis.
Left common carotid arteries were denuded of endothelium using a 250-μm polytetrafluoroethylene filament.
Inhibition of tumor growth by targeting tumor endothelium using a soluble vascular endothelial growth factor receptor.
In vivo brain imaging using a portabler3.
The endothelium-dependent agonist ACh produced marked relaxation (<20% residual contraction), suggestive of a fully functioning, undamaged endothelium using this methodology.
Using human brain endothelium in a stroke model, Cheng and coworkers [ 10] reported that APC had a direct antiapoptotic effect on hypoxic brain endothelium that required binding to EPCR and PAR1 activation.
The endothelium was visualized using a similar immunostaining protocol for identification of smooth muscle cells.
The interaction between lymphocytes and brain endothelium is a central pathogenetic event in CNS autoimmunity and, thus, represents an important focus of investigation.
Endothelium and endothelium independent vasodilation was assessed by using a high-resolution ultrasound system with a 7 10-MHz linear array transducer (Acuson Aspen; Acuson Corp).
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