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Ten-µm brain cryosections were prepared, fixed in 75% ethanol for 30s.
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Embryonic brains and cryosections were prepared as previously described.
Brains were removed, shock frozen in liquid nitrogen, and cryosections were prepared.
Cryosections were prepared for immunohistochemistry (IHC).
Ten-micron cryosections were prepared for incubation with human serum.
For each group, cryosections were prepared from four tumors harvested from two mice.
Ultrathin cryosections were prepared according to [40].
The contralateral hemisphere was fixed and cryosections were prepared.
Ultrathin cryosections were prepared according to Tokuyasu [34] and picked up on nickel grids.
Cryosections were prepared and placed on slides as described in the preceding section.
Intact hearts were frozen in OCT embedding compound (Tissue Tek, Torrance, CA), and 10 µm cryosections were prepared.
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