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For quantification of endogenous fibronectin (FN) leakage across the BBB brain cryosections were performed as described above on day 50 p.i. of 6 double and 9 single transgenic Tie-2 tTA//TRE claudin-1 mice (lines 23949 and 23974).
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Measurements of wound area and ventricle area on cryosections were performed with the Fiji distribution of the ImageJ (NIH) software.
Cryosections were performed as follows: 5d.p.f.
Paraffin sections and cryosections were performed as described (47, 48).
The hybridizations on cryosections were performed as described by Hidalgo-Sánchez et al. (2005).
Cryosections were performed on skeletally mature mice (P100) in order to preserve β-galactosidase staining.
Immunocytochemical studies on 8-μm cryosections were performed as described previously [ 32].
In situ hybridisation on cryosections was performed as previously described [25].
ISH on PFA-fixed cryosections was performed according to standard protocols for mouse [75], chicken, zebrafish [76] and axolotl [77].
TUNEL staining on cryosections was performed as described before [35] using TUNEL-TMR-Red kit (Roche, cat # 1966006001).
Immunohistochemistry on cryosections was performed as described previously.
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