Sentence examples for brain cells were stained from inspiring English sources

Brain cells were stained for FACS analysis according to standard protocols in cold PBS containing 2% FCS and 0.01% sodium azide (FACS buffer) with the following Abs (all from BD Biosciences, Le Pont de Claix, France): FITC-labeled anti CD45, APC-labeled CD4, phycoerythrin (PE -labeled anti-CD8α, PE -labeledanti-CD8α and APE-labeled anti-GR11b.

The brain cells were stained with FITC/anti-CD3 and allophycocyanin (APC /anti-CD4 APC /anti-CD4Bioscience).

The brain cells were stained for BrdU by immunohistochemistry and the number of BrdU-positive cells was evaluated using microscopy.

To identify the major normal brain cell types, the normal brain cells were stained with mouse CD11b-PE-Cy7 antibodies (BD Pharmingen) or primary antibodies against MAP2 (Abcam), GFAP (Abcam), nestin (Sigma) and MBP (Abcam) and detected with DyLight 488-conjugated secondary antibodies (BioLegend, San Diego, CA).

Finally, positive cells were stained brown with diaminobenzidine, and brain sections were counterstained with toluidine blue.

Cells were stained for surface PD-L1 expression, followed by intracellular staining for p-STAT3.

After fixation, cells were stained with anti-BrdU antibody.

The nuclei of the cells were stained with DAPI.

Cells were stained for JEV antigen by protocol described earlier30.

Isolated primary tumour cells were stained with SA-β gal.

Erythroid cells were stained with PKH26 and Hoechst 33342.