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Nuclear extracts of brain cells were prepared according to Semenza and Wang (1992) [67] with some modifications after Soitamo et al. (2001) [68].
Dissociated brain cells were prepared as described [44].
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Crude extracts of budding yeast, porcine brain or MovS6 (murine neuroglial) cells were prepared and incubated for 30 minutes with 6AP beads, GA beads or control beads.
These differences may be due to culture conditions, ages of the embryos/fetuses, or the brain areas from which the cells were prepared.
Briefly, primary cultures of glial cells were prepared from the brains of 1 2-day-old Wistar rats and oligodendrocytes were mechanically removed from the flasks after 6–8 days.
Primary cultures of microglial cells were prepared from the brains of newborn C57/BL6 mice as previously described [ 43].
Astrocytes and microglial cells were prepared from the brains of neonatal (postnatal day 3) mice as previously described (Dvoriantchikova et al., 2011; Barakat et al., 2012; Santos et al., 2014).
Microglial cells were prepared from newborn rat brain essentially as described [13].
Primary human microglial cells were prepared from embryonic human brains of 12 15 weeks gestation as previously described [21].
Cells were prepared in quadruplet wells.
Cells were prepared as previously described [14].
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