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To discern what was different, physiologically, about the stress-resistant mice, the scientists looked at brain cells using stains and other techniques.
DNA damage was assessed in peripheral blood, liver and brain cells using the comet assay (tail moment).
To complement the findings from humans and in vivo experimentation, my laboratory group has investigated the effects of repeated trauma in cultured brain cells using a model of stretch-induced mechanical injury in vitro.
We analysed the dynamic behaviour of Spd-2 GFP Spd-2 GFPn at centroromes in mitotic Drosophila larval brain cells usinGFP Cnnescence recovery atter photobleacentrosomes).
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Because active brain cells use more oxygen, they can be seen as hot spots against a background of less active cells.
Some have suggested that it is blood sugar, which brain cells use as their main energy source and cannot do without for even a few minutes.
In one such approach, researchers surgically remove brain cells, use viruses to transfer genes to the cells, and then graft them back into the animal's brain tissue.
Larger cells, like the brain cells used in the experiments, are then easier to see using traditional microscopy techniques and may reveal insights into disease progression previously beyond the resolution of our best scopes.
Weeks of stress cause reversible damage to neuronal dendrites (the small "arms" that brain cells use to communicate with each other), and months of stress can permanently destroy neurons.
However, the presence of ApoB-100 protein was demonstrated in the membrane microdomains of primary brain endothelial cells using mass spectrometry [ 33].
In the past few years, some have jury-rigged ways to excite brain cells by using light; one technique used at Yale made headless fruit flies flap away.
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