Sentence examples for brain cDNA using the from inspiring English sources

The coding sequence for feline CD34 was isolated from brain cDNA using the polymerase chain reaction (PCR) with oligonucleotides designed to conserved regions of known CD34 gene sequences as primers.

The Ct values were measured and converted to ng total brain cDNA using the corresponding standard curves: 10 ∧ (Ct value - intercept)/slope [ng total brain cDNA].

Murine PrPC was amplified from total brain cDNA, using the primers SY6 and SY7 (Table S1), which introduces the BamHI and the SalI cleavage sites, respectively.

Spire1C was amplified from mouse brain cDNA using the sequence of NM_194355 as a reference.

Finally, the entire coding sequence of pike elovl5 was amplified from brain cDNA using the high fidelity Pfu Turbo DNA polymerase (Stratagene, Agilent Technologies, Cheshire, UK) by performing a nested PCR.

Standard curves relating amplification cycle with initial template quantity were generated using serial dilutions of specific RT-PCR products for each gene (obtained from brain cDNA using the same primers) and included in all quantification plates, with qPCR efficiency ranging between 85 97% with R > 0.98.

RT-PCR from mouse brain cDNA using specific primers for TG6 and also the other members of the TG family revealed expression of TG1, TG2, TG3, TG5, TG6 and TG7 (Fig.  3a).

Human MAPK14/P38α gene was amplified from whole brain cDNA using primers 5'-ATGTCTCAGGAGAGCCCACGTTCTAC-3' and 5'-TCAGGACTCCATCCTTCTTGGTCAAG-3'.

Subclones of PNKD-L were obtained by RT PCR from human brain cDNA using Expanded High Fidelity PCR (Roche).

Complementary DNAs for N-terminally HA-tagged E2F-1 were amplified by PCR from brain cDNA using primer sets designed in accordance with known sequences (INSD accession number: AF516106) and subcloned into the BamHI- XbaI sites of pcDNA3 (Invitrogen).

cDNAs encoding rat Azin1 and Odc were amplified from rat brain cDNA using primers (5′-ACGGGATCCATGAAAGGATTTATTGACGATG-3′ and 5′-ACTCTCGAGCTAAAGAAGCGTTAATGCC-3′ and 5′-CGAGAATTCACCATGGGCAGCTTTAC-3′ and 5′-TAAGGATCCAGGTAAGAGCTACAAGAATG-3′, respectively), digested, and cloned into corresponding restriction sites of lentiviral vector pRRL.SIN.CPPT.CMV.IRES.GFP.WPRE.