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All brain cDNA samples from different seasonal time points were synthesized in parallel.
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In this study, to determine whether viral escape at the immunodominant SIV Gag KP9 epitope could be detected in the CNS of animals with the Mane-A*10 allele that developed SIV CNS disease with high levels of vRNA in brain, cDNA samples prepared from brain RNA from the six Mane-A*10 positive animals with SIV encepositive were bulk PCR animalsed and sequenced.
One run of Illumina sequencing was done for each F1 female brain cDNA sample.
Wolf et al. 2010 [ 44] pyrosequenced barcoded cDNA samples from brain of 11 wild caught sub-adult crows (~2 years old) on a Roche 454 FLX sequencer.
cDNA samples from mouse brain tissue were dissected at the times indicated from NIH Swiss dams.
cDNA samples from human fetal and adult postmortem brain tissue were obtained commercially (BioChain Institute, Hayward, CA and Clontech, Mountain View, CA).
Here we investigate these issues using a panel of 20 candidate reference genes and 15 cDNA samples from Drosophila heads that are associated with brain aging or neurodegeneration.
Each cDNA sample was derived from mRNA pooled from each litter, which was composed of several embryos (e.g., three cDNA samples from wild-type mice; six cDNA samples from BB/− mice; eight cDNA samples from XB mice).
No mutation was observed in cDNA samples from other tissues.
cDNA samples from two unaffected individuals were used as controls.
A kinectin cDNA clone containing a 4.7-kilobase insert was isolated from an embryonic chick brain cDNA library by immunoscreening with a panel of monoclonal antibodies.
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