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The double mutants aapJ::ΩSp braC::mTn5 (LMB210) and aapJ::ΩSp braD::mTn5 (LMB211) were generated in the S. meliloti 2011 background as described in Materials and Methods.
BRAD V2.0 is a substantially improved version of BRAD V1.0.
In total, about 4 Gb of data have been collected in BRAD V2.0.
First, B. rapa genome annotation information in GFF3 format was retrieved from BRAD (Brapa_gene_v1.2.gff).
BRAD V2.0 has five main sections: Browse, Search, Tools, Download and Links.
BRAD V1.0 has now been updated to V2.0 to include Brassicaceae genome sequences that have been released recently.
In BRAD V2.0, a new section has been incorporated that shows genomic synteny and micro-fragmental synteny between any two Brassicaceae species.
The updated BRAD V2.0 will be a valuable resource for research into comparative genomics, plant evolution and molecular biology, as well as for breeders of Brassicaceae crops.
In BRAD V1.0, datasets of genome and gene sequences, gene annotations, non-coding RNAs, transposable elements, genetic markers and linkage maps of B. rapa were provided (15, 16).
The GBrowse_syn (16) tool for visualizing synteny or collinear genomic regions among multiple genomes can be accessed from the Tools section of BRAD 2.0.
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