In addition, Keyes reported the complications of brachial artery puncture in 2% of cases, and that the IV catheter fell out or was infiltrated within 1 h of placement occur in 8% of emergency department patients with antecubital lines [7].
A blood sample was taken by brachial vein puncture to a hematocrit capillary (ca. 70 µl).
At the time of the swapping, a drop of blood (20 50 µl) was collected from each nestling by brachial vein puncture and stored in ethanol.
Twenty-five dafterfthe thatchinging of the first chick in the brood, i.e. just before fledging, we collected a blood sample from the chicks by brachial vein puncture.
After overnight fast, ~200 µl whole blood was collected by submandibular vein lancet bleed (glucose) or brachial sinus puncture (remaining assays).
After overnight fast, ~200 μl whole blood was collected by submandibular vein lancet bleed (glucose) or brachial sinus puncture (remaining assays).
At the first capture, we obtained a small amount of blood (20 μl) by brachial vein puncture and stored it in 500 μl of Queen's Lysis Buffer (QLB) [ 45].
Antecubital venous access procedures can be complicated by brachial artery punctures, hematoma formation, brachial nerve injury, severe arm pain post procedure, and catheter malposition [4, 5, 7, 11].
At time of banding, a small blood sample was collected by brachial veni-puncture from nestlings (n = 164) and fledglings that were still dependent on their parents (n = 48), and stored in Queens- or Longmire's lysis buffer for paternity analyses (see below).
Blood samples were collected by puncturing the brachial vein and the puncturing site was accurately disinfected.
