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The effect of sequence length on RMS calculations can be visualised by comparison of the degree of scatter of dinucleotide frequencies of human mRNA sequences (mean length of approximately 2463 bases) with that of the much longer DNA sequences (50,000 bps; Figures 1A, 1D, 2A, 2B).
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RAPD and ISSR primers produced amplicons with a range of 250 4000 bps (Figure 6).
A change in electrophoretic property of RIG-I was also observed with shR9 and poly(I∶C) of ∼105 bps (Figure 6A).
Unlike MRC-5 fibroblastsblands anormalmal human dermal fibroblasts (NHDF), fibrocytes and macrophages had no detectable staining for CD90, CD248 (endosialin), FAP, TE-7, cellular fibronectin, or hyaluronan (HA-BP) (Figures 1, 7, and 8).
The size of smallest genomic island was 4505 bp while largest was 78290 bp (Figure 2, Additional file 1: Table S3).
PCR showed an amplification of 700 bp (Figure 1C) as expected with a putative molecular weight of approximately 22.86 kDa as determined by SDS-PAGE (Figure 1B).
The gene for the VHH domain of about 400 bp (Figure 2B) was amplified with nested primers PMCF, BACK-1, and BACK-2 using as template the 600 bp PCR fragment.
IDEF1BS motifs and BREU-TATA motif 1 were frequently co-localized with a separation of 50 bp (Figure 3D), suggesting that the transcription factors binding to IDEF1BS and BREU-TATA motif 1 may interact.
qpl6-1 and qph6-1 thareare mapped to the same bin (bin3619); OsIAA23 (Jun et al.2011) is near to qpl6-2 with peak value within the ph6 interval (6927624-29906021 bp) (Figure 4B).
The product sizes are as follows: CrTflox: 548 bp; CrT+: 422; CrT−: 346 bp (Figure 1C).
The PCR product length was approximately 400 bp (figure 5a) and was subsequently sequenced (figure 5b).
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