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The minimum read length was 45 bp with a minimum Sanger quality score of 35.
The median size of PSRs is 123 bp with a minimum of 25 bp.
Each region in the reference genomes longer than 25 bp with a minimum coverage of 9 reads was reported.
Global DNA alignments of chromosomal segments were performed using LAGAN [ 59] in a window of 100 bp with a minimum identity of 70%.
The average overlap between amplicons is 210 bp, with a minimum overlap of 71 bp (between Amplicons 7 and 8) and a maximum overlap of 338 bp.
Window size was 20 bp, with a minimum of 18 bp required to meet the criteria for either gain or loss.
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The mean CNV length was 110,455 bp with a maximum and minimum length of 4,580,011 bp and 10,046 bp respectively.
We located di-, tri-, and tetra-nucleotide SSRs with lengths less than 50 bp and with a minimum of 4 contiguous repeating units, which provided a large number of candidate SSRs.
A threshold alignment score of E-10 was used to filter the results, and only the alignments of at least 100 bp and with a minimum of 95% of identity between the sequences were retained.
The average length of the BESs was 683 bp with a maximum of 1112 bp and minimum of 101 bp.
The array comprises 385,585 probes semi-tiled across the genome at a 4 bp interval spacing with a minimum probe length of 45 bp, and a maximum length of 85 bp.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com